SKLPT imaging: Efficient in vivo pre-evaluation of genome-editing modules using fluorescent protein with peroxisome targeting signal.
Konno R, Tanaka H, Kodama Y
Biochem Biophys Res Commun. 2018 Sep 3;503(1):235-241. doi: 10.1016/j.bbrc.2018.06.008. Epub 2018 Jun 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186720 | pDONR/Zeo-sfGFP-SKL | Gateway entry vector encoding sfGFP with the two peroxisome-targeting signals (out of frame). |
| 186721 | pGWT35S-sfGFP-SKL-Target1 (Mpphot) | Transient expression vector for SKLPT imaging for target 1 (Mpphot) in plant cells (Positive control vector). |
| 186722 | pGWT35S-sfGFP-SKL-Target2 (Mpphot) | Transient expression vector for SKLPT imaging for target 2 (Mpphot) in plant cells (Negative control vector). |
| 186723 | pGWT35S-Cas9 | Transient expression of Cas9 in plant cells. |
| 186726 | pMpGE_En03-sgRNA_Target1 (Mpphot) | Gateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells. |
| 186727 | pMpGE_En03-sgRNA_Target2 (Mpphot) | Gateway entry vector for sgRNA (target 2: Mpphot [negative control]). Transient expression of sgRNA (target 2: Mpphot) in plant cells. |
| 186728 | pMpGE010-Target1 (Mpphot) | Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation). |
| 186729 | pMpGE010-Target2 (Mpphot) | Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation). |