SKLPT imaging: Efficient in vivo pre-evaluation of genome-editing modules using fluorescent protein with peroxisome targeting signal.

Konno R, Tanaka H, Kodama Y
Biochem Biophys Res Commun. 2018 Sep 3;503(1):235-241. doi: 10.1016/j.bbrc.2018.06.008. Epub 2018 Jun 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
186729	pMpGE010-Target2 (Mpphot)	Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).
186728	pMpGE010-Target1 (Mpphot)	Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).
186727	pMpGE_En03-sgRNA_Target2 (Mpphot)	Gateway entry vector for sgRNA (target 2: Mpphot [negative control]). Transient expression of sgRNA (target 2: Mpphot) in plant cells.
186726	pMpGE_En03-sgRNA_Target1 (Mpphot)	Gateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells.
186723	pGWT35S-Cas9	Transient expression of Cas9 in plant cells.
186722	pGWT35S-sfGFP-SKL-Target2 (Mpphot)	Transient expression vector for SKLPT imaging for target 2 (Mpphot) in plant cells (Negative control vector).
186721	pGWT35S-sfGFP-SKL-Target1 (Mpphot)	Transient expression vector for SKLPT imaging for target 1 (Mpphot) in plant cells (Positive control vector).
186720	pDONR/Zeo-sfGFP-SKL	Gateway entry vector encoding sfGFP with the two peroxisome-targeting signals (out of frame).