Anaerobic production of medium-chain fatty alcohols via a beta-reduction pathway.
Mehrer CR, Incha MR, Politz MC, Pfleger BF
Metab Eng. 2018 Jul;48:63-71. doi: 10.1016/j.ymben.2018.05.011. Epub 2018 May 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215559 | pMP11 | pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin |
| 215560 | pgRNA | Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR |
| 215649 | ptrc99a-pVhb-tdTER-fdh | pBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoter |
| 215650 | pACYC-pVhb-vfFadBA | pACYC Origin, CmR, with vfFadBA gene under Vhb promoter |
| 215651 | pBTRCk-pVhb-maACR | pBBR1 Origin, KanR, with maACR gene under Vhb promoter |