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Spectrally Resolved Fiber Photometry for Multi-component Analysis of Brain Circuits.

Meng C, Zhou J, Papaneri A, Peddada T, Xu K, Cui G
Neuron. 2018 May 16;98(4):707-717.e4. doi: 10.1016/j.neuron.2018.04.012. Epub 2018 May 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
141236pAAV-Ef1a-FAS-NES-jRGECO1a-WPREAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
141237pAAV-Ef1a-FAS-GCaMP6f-WPREAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)
216275pAAV-hSyn-DIO-GCaMP6f-WPREAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f
216276pAAV-hSyn-DIO-NES-jRGECO1a-WPREAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1a
216277pAAV-hSyn-FAS-GCaMP6f-WPREAAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

Antibodies from Article