Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for precision tumor modeling.
Oldrini B, Curiel-Garcia A, Marques C, Matia V, Uluckan O, Grana-Castro O, Torres-Ruiz R, Rodriguez-Perales S, Huse JT, Squatrito M
Nat Commun. 2018 Apr 13;9(1):1466. doi: 10.1038/s41467-018-03731-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136405 | pDONR-U6gRNA-PGKpuro2ABFP | Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP. |
| 136409 | pDONR-U6gRNA-PGKpuro2APDGFB | Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB. |
| 136413 | pKLV-gRNA-Bcan-Ntrk1_4 | Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP. |
| 136415 | pKLV-gRNA-Myb-Qk_1 | Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP. |