Conditional gene knockout and reconstitution in human iPSCs with an inducible Cas9 system.
Wu M, Liu S, Gao Y, Bai H, Machairaki V, Li G, Chen T, Cheng L
Stem Cell Res. 2018 May;29:6-14. doi: 10.1016/j.scr.2018.03.003. Epub 2018 Mar 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133353 | human ETV6 gRNA-1 | human ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon. |
| 133403 | human ETV6 gRNA-3 | human ETV6 gRNA-3 is a 20-nt gRNA expression plasmid targeting the second ETV6 exon. |
| 133404 | human ETV6 gRNA-4 | human ETV6 gRNA-3 and 4 is a pair of gRNAs. Four ETV6 gRNA plasmids used the same backbone(Addgene plasmid#41824). ETV6 gRNA-4 target the second ETV6 exon. |