Engineering Introns to Express RNA Guides for Cas9- and Cpf1-Mediated Multiplex Genome Editing.
Ding D, Chen K, Chen Y, Li H, Xie K
Mol Plant. 2018 Apr 2;11(4):542-552. doi: 10.1016/j.molp.2018.02.005. Epub 2018 Feb 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158151 | pRGEB33 | Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing. |
| 158152 | pRGEB34 | Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing |
| 158153 | p32Fn | Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing. |
| 158154 | p32Lb | Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing. |
| 158155 | p33Fn | Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing. |
| 158156 | p33Lb | Expression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing. |