CRISPR/Cas9 screening using unique molecular identifiers.

Schmierer B, Botla SK, Zhang J, Turunen M, Kivioja T, Taipale J
Mol Syst Biol. 2017 Oct 9;13(10):945. doi: 10.15252/msb.20177834. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
196709	lentiGuide-Puro.3xBsmBI	Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice
196710	lentiGuide-Puro-Chen-tracrRNA_2xBsmBI	Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice
196713	lenti-Cas9-sgHPRT1	CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG