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A Viral Toolbox of Genetically Encoded Fluorescent Synaptic Tags.

Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man HY, Han X
iScience. 2020 Jun 30;23(7):101330. doi: 10.1016/j.isci.2020.101330. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
125691AAV-EF1A-PSD95.FingR-eGFP-CCR5TCGlobal labeling of excitatory synapses (green fluorescence)
125692AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TCGlobal labeling of inhibitory synapses (green fluorescence)
125693AAV-Syn-PSD95.FingR-eGFP-CCR5TCGlobal labeling of excitatory synapses (green fluorescence)
125694AAV-Syn-PSD95-mCherryExogenous expression of PSD95 (red fluorescence)
125695AAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTCGlobal labeling of inhibitory synapses (red fluorescence)
125696AAV-mRuby2-Gephyrin.FingR-IL2RGTCGlobal labeling of inhibitory synapses (red fluorescence)
126212MSCV-Syn-PSD95.FingR-eGFPLabeling of excitatory synapses (green fluorescence) in dividing cells
126215AAV-EF1A-DIO-mRuby2Labeling of cre-expressing neurons (red fluorescence)
126216AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TCLabeling of excitatory synapses (green fluorescence) in cre-expressing neurons
126217AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TCLabeling of inhibitory synapses (green fluorescence) in cre-expressing neurons
126218Lenti-CamKII-PSD95.FingR-eGFP-CCR5TCGlobal labeling of excitatory synapses (green fluorescence)

Antibodies from Article