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Pointing in the Right Direction: Controlling the Orientation of Proteins on Nanoparticles Improves Targeting Efficiency.

Yong KW, Yuen D, Chen MZ, Porter CJH, Johnston APR
Nano Lett. 2019 Mar 13;19(3):1827-1831. doi: 10.1021/acs.nanolett.8b04916. Epub 2019 Feb 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
125264pTrcHIS-7D12Gln13TAGExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF
125265pTrcHis-7D12Gly42TAGExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzF
125266pTrcHis-7D12Asp73TAGExpresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzF
125267pTrcHis-7D12CTermTAGExpresses 7D12 anti-EGFR nanobodies with UAG codon at c-terminus for synthetic amino acid in combination with pEVOL-pAzF
125268pTrcHIS-wt7D12Expresses wild type 7D12 anti-EGFR nanobodies

Antibodies from Article