Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels.
Depardieu F, Bikard D
Methods. 2019 Aug 1. pii: S1046-2023(18)30497-3. doi: 10.1016/j.ymeth.2019.07.024.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124768 | pFD115 | pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector |
| 124769 | pFD116 | pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector |
| 125258 | FD1 | Derivative of MG1655 used for the screening of clones able to survive the bad seed toxicity of dCas9, while still efficiently silencing the expression of mcherry |
| 125546 | pFD152 | derivative of pFD116 with an optimized RBS for E. coli |