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RNA exploits an exposed regulatory site to inhibit the enzymatic activity of PRC2.

Zhang Q, McKenzie NJ, Warneford-Thomson R, Gail EH, Flanigan SF, Owen BM, Lauman R, Levina V, Garcia BA, Schittenhelm RB, Bonasio R, Davidovich C
Nat Struct Mol Biol. 2019 Mar;26(3):237-247. doi: 10.1038/s41594-019-0197-y. Epub 2019 Mar 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
125161pFB1.HMBP.PrS.EZH2Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
125162pFB1.HMBP.PrS.SUZ12Expresses human SUZ12 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
125163pFB1.HMBP.PrS.EEDExpresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
125164pFB1.HMBP.PrS.RBBP4Expresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
125165pFB1.HMBP.PrS.AEBP2Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
125166pFB1.HMBP.PrS.JARID2Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
125167pFB1.HMBP.PrS.PHF1Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
125168pFB1.HMBP.PrS.PHF19Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
125169pFB1.HMBP.PrS.MTF2Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
125170pFB1.HMBP.PrS.EPOPExpresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
125171pFBOH-MHL.EZH2Expresses human EZH2 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.
125172pFBOH-MHL.SUZ12Expresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.
125173pFBOH-MHL.RBBP4Expresses human RBBP4 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.
125174pFBOH-MHL.EEDExpresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Antibodies from Article