Transcriptional cofactors display specificity for distinct types of core promoters.
Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A
Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. Epub 2019 May 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 125149 | pSTAP-seq_fly-4xUAS | Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells |
| 125150 | pSTAP-seq_human-4xUAS | Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells |
| 125151 | pSTAP-seq_fly_spike-in | Vector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cells |
| 125152 | pSTAP-seq_human_spike-in | Empty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cells |
| 125153 | pAGW-dpse-GAL4-DBD | Gateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CP |
| 125154 | pGL3_4xUAS_CP-candidate_luc+ | STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins |
| 125156 | pENTR_hEMSY | Gateway entry clone |
| 125159 | pENTR_Trf2 | Gateway entry clone |