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Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins.

Qiao J, Li W, Lin S, Sun W, Ma L, Liu Y
Commun Biol. 2019 May 3;2:161. doi: 10.1038/s42003-019-0402-x. eCollection 2019. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
124890pCold CL7-Cas9obtaining full Cas9 RNP
124891pCold CL7-Cas12aobtaining full Cas12a RNP

Antibodies from Article