von Willebrand factor self-association is regulated by the shear-dependent unfolding of the A2 domain.

Zhang C, Kelkar A, Neelamegham S
Blood Adv. 2019 Apr 9;3(7):957-968. doi: 10.1182/bloodadvances.2018030122. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
124794	pcDNA3.1-WT-VWF	Expresses multimeric human VWF with C-terminal c-Myc tag
124823	pcDNA3.1-deltaA1-VWF	Expresses multimeric human VWF protein without A1 domain. This construct has no tag.
124824	pcDNA3.1-deltaA1-Lock-VWF	Expresses multimeric human VWF protein without A1 domain and Lock A2 domain. This protein has C-terminal c-Myc tag.
124825	pcDNA3.1-deltaA1-D1498A-VWF	Expresses multimeric human VWF protein without A1 domain and a D1498A mutation in A2 domain. This protein has C-terminal c-Myc tag.
124826	pcDNA3.1-deltaA1-R1597W-VWF	Expresses multimeric human VWF protein without A1 domain and a R1597W mutation in A2 domain. This protein has C-terminal c-Myc tag.
124827	pCS-CG-WT-Cer-VWF	Expresses multimeric human VWF-ECFP fusion protein. The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.
124828	pCS-CG-Lock-Cer-VWF	Expresses multimeric human VWF-ECFP fusion protein with A2-Lock domain The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.