Rewiring bacterial two-component systems by modular DNA-binding domain swapping.
Schmidl SR, Ekness F, Sofjan K, Daeffler KN, Brink KR, Landry BP, Gerhardt KP, Dyulgyarov N, Sheth RU, Tabor JJ
Nat Chem Biol. 2019 Jul;15(7):690-698. doi: 10.1038/s41589-019-0286-6. Epub 2019 May 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124712 | pSR305 | Expression of uhpB under Ptac |
| 124713 | pSR348 | Expression of narX under Ptac |
| 124714 | pKD251 | Expression of torS under Ptac and torT under PD59_luxR |
| 125069 | pKD276 | Expression of SO_0621 under Ptac |
| 125070 | pKD278 | Expression of SO_2192 under Ptac |
| 125071 | pKD280 | Expression of SO_4387 under Ptac |
| 125072 | pKD282 | Expression of SO_4427 under Ptac |
| 125073 | pKD284 | Expression of SO_4488 under Ptac |
| 125074 | pKD286 | Expression of SO_4622 under Ptac |
| 125075 | pKD288 | Expression of SO_4638 under Ptac |
| 125076 | pSR62 | Expression of ompR under PLtetO-1, output promoter PompF146 |
| 125077 | pKD250.2 | Expression of torR under PLtetO-1, output promoter PtorCAD129 |
| 125078 | pSR353 | Expression of narL under PLtetO-1, output promoter PdcuS77 |
| 125079 | pSR300.3 | Expression of uhpA under PLtetO-1, output Promoter PuhpT99 |
| 125080 | pSR40.13 | Expression of ompR(1-118aa)-ccaR(115-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172 |
| 125081 | pSR40.14 | Expression of ompR(1-122aa)-ccaR(119-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172 |
| 125082 | pSR40.15 | Expression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172 |
| 125083 | pSR40.16 | Expression of ompR(1-140aa)-ccaR(130-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172 |
| 125084 | pSR40.18 | Expression of ompR(1-150aa)-ccaR(140-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172 |
| 125085 | pSR40.25 | Expression of ompR(1-110aa)-ccaR(107-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172 |
| 125086 | pSR40.26 | Expression of ompR(1-113aa)-ccaR(110-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172 |
| 125087 | pSR40.28 | Expression of ompR(1-138aa)-ccaR(128-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172 |
| 125088 | pSR40.29 | Expression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172 |
| 125089 | pSR40.30 | Expression of ompR(1-142aa)-ccaR(132-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172 |
| 125090 | pSR240 | Expression of ompR(1-137aa)-psdR(134-237aa) chimera under PLtetO-1, output Promoter PpsdA110 |
| 125091 | pSR241 | Expression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181 |
| 125092 | pSR276.4 | Expression of ompR(1-122aa)-psdR(119-237aa) chimera under PLtetO-1, output Promoter PpsdA110 |
| 125093 | pSR284 | Expression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143 |
| 125094 | pKD263 | Expression of torR(1-134aa)-ccaR(127-234aa), ompR137 chimera under PLtetO-1, output Promoter PcpcG2-172 |
| 125095 | pKD265 | Expression of torR(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110 |
| 125096 | pKD266 | Expression of torR(1-134aa)-yxdJ(132-229aa), ompR137 chimera under PLtetO-1, output Promoter PyxdJ181 |
| 125097 | pKD275 | Expression of SO_0622(1-129aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110 |
| 125098 | pKD277 | Expression of SO_2193(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110 |
| 125099 | pKD279 | Expression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110 |
| 125100 | pKD281 | Expression of SO_4428(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110 |
| 125101 | pKD283.2 | Expression of SO_4487(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110 |
| 125102 | pKD285 | Expression of SO_4623(1-124aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110 |
| 125103 | pKD287 | Expression of SO_4637(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110 |
| 125104 | pSR357.6 | Expression of narL(1-126aa)-ydfI(121-213aa) chimera under PLtetO-1, output Promoter PydfJ115 |
| 125105 | pSR357.9 | Expression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115 |
| 125106 | pSR357.10 | Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115 |
| 125107 | pSR357.12 | Expression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115 |
| 125108 | pSR357.20 | Expression of narL(1-154aa)-ydfI(148-213aa) chimera under PLtetO-1, output Promoter PydfJ115 |
| 125109 | pSR357.24 | Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115 |
| 125110 | pSR357.27 | Expression of narL(1-120aa)-ydfI(115-213aa) chimera under PLtetO-1, output Promoter PydfJ115 |
| 125111 | pSR357.28 | Expression of narL(1-122aa)-ydfI(117-213aa) chimera under PLtetO-1, output Promoter PydfJ115 |
| 125112 | pSR358 | Expression of narL(1-131aa)-liaR(127-211aa) chimera under PLtetO-1, output Promoter PliaI121 |
| 125113 | pSR358.2 | Expression of narL(1-126aa)-liaR(122-211aa) chimera under PLtetO-1, output Promoter PliaI121 |
| 125114 | pSR358.4 | Expression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121 |
| 125115 | pSR359.4 | Expression of narL(1-159aa)-uhpA(142-129aa) chimera under PLtetO-1, output Promoter PuhpT99 |
| 125116 | pSR322 | Expression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115 |
| 125117 | pSR322.2 | Expression of uhpA(1-118aa)-ydfI(121-213aa), narL126 chimera under PLtetO-1, output Promoter PydfJ115 |
| 125118 | pSR322.3 | Expression of uhpA(1-121aa)-ydfI(124-213aa), narL129 chimera under PLtetO-1, output Promoter PydfJ115 |
| 125119 | pSR322.4 | Expression of uhpA(1-141aa)-ydfI(153-213aa), narL159 chimera under PLtetO-1, output Promoter PydfJ115 |