Engineering an NADPH/NADP(+) Redox Biosensor in Yeast.
Zhang J, Sonnenschein N, Pihl TP, Pedersen KR, Jensen MK, Keasling JD
ACS Synth Biol. 2016 Dec 16;5(12):1546-1556. doi: 10.1021/acssynbio.6b00135. Epub 2016 Jul 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124708 | pCfB3236 | Expresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS416 |
| 126750 | pCfB4170 | Expresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS413 |
| 126751 | pCfB4929 | Expresses pTRX2_5xUAS-ALD6-tADH1 in yeast from plasdmid pRS413 |
| 126752 | pCfB4613 | Integrate pTRX2_5xUAS-CDC14-tADH1 into yeast genome EasyClone site X-4 with SpHIS5 marker |