The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage.
Koerver L, Papadopoulos C, Liu B, Kravic B, Rota G, Brecht L, Veenendaal T, Polajnar M, Bluemke A, Ehrmann M, Klumperman J, Jaattela M, Behrends C, Meyer H
EMBO Rep. 2019 Aug 21:e48014. doi: 10.15252/embr.201948014.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124665 | pcDNA5FRT/TO-Strep/HA-UBE2QL1 | Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells |
| 124666 | pcDNA5FRT/TO-UBE2QL1-Strep/HA | Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells |
| 124667 | pcDNA5FRT/TO-UBE2QL1-GFP | Expresses UBE2QL1 with C-terminal GFP tag in mammalian cells |
| 124668 | pcDNA5FRT/TO-Strep/HA-UBE2Q2 | Expresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cells |
| 124669 | pcDNA5FRT/TO-Strep/HA-UBE2J1 | Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells |
| 129513 | pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA | Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells |