Measuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor.
Monteiro F, Hubmann G, Takhaveev V, Vedelaar SR, Norder J, Hekelaar J, Saldida J, Litsios A, Wijma HJ, Schmidt A, Heinemann M
Mol Syst Biol. 2019 Dec;15(12):e9071. doi: 10.15252/msb.20199071.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124582 | pCggRO-reporter | Reporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine) |
| 124583 | pTEF6-7 | Reporter plasmid with constitutively expressed mCherry and YFP (eCitrine) |
| 124584 | pHO_pTEFmut7_CggR_ble | Plasmid for genomic integration of CggR repressor regulated by the pTEFmut7 promoter |
| 124585 | pHO_pTEFmut7_CggR_R250A_ble | Plasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoter |