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Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC domain are required for protein binding and when mutated lead to delayed peduncle and flag leaf senescence.

Harrington SA, Overend LE, Cobo N, Borrill P, Uauy C
BMC Plant Biol. 2019 Sep 18;19(1):407. doi: 10.1186/s12870-019-2022-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
124572pGWB21_TaNAM-A1Expresses full-length TaNAM-A1 with an N-terminal 10x Myc tag under the 35s promoter.
124573pGWB12_TtNAM-B1Expresses full-length TtNAM-B1 with an N-terminal FLAG tag under the 35s promoter.
124574pCR8_TaNAM-A1TaNAM-A1 CDS in the Gateway-compatible pCR8 vector.
124575pCR8_TtNAM-B1TtNAM-B1 CDS in the Gateway-compatible pCR8 vector.
124576pCR8_TaNAM-A1(1-217)NAC domain (1-217) of TaNAM-A1 in the Gateway-compatible pCR8 vector.
124577pCR8_TtNAM-B1(1-215)NAC domain (1-215) of TtNAM-B1 in the Gateway-compatible pCR8 vector.

Antibodies from Article