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Enabling large-scale genome editing at repetitive elements by reducing DNA nicking.

Smith CJ, Castanon O, Said K, Volf V, Khoshakhlagh P, Hornick A, Ferreira R, Wu CT, Guell M, Garg S, Ng AHM, Myllykallio H, Church GM
Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
124447dABEExpresses dead Cas9 (dCas9)-ABE7.10
124449dCBE4-gamExpresses dead Cas9 (dCas9)-CBE4-gam
124450pSB700 HL1gR4Expresses gRNA HL1 gR4 in the pSB700 backbone

Antibodies from Article