Enabling large-scale genome editing at repetitive elements by reducing DNA nicking.
Smith CJ, Castanon O, Said K, Volf V, Khoshakhlagh P, Hornick A, Ferreira R, Wu CT, Guell M, Garg S, Ng AHM, Myllykallio H, Church GM
Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
124447 | dABE | Expresses dead Cas9 (dCas9)-ABE7.10 |
124449 | dCBE4-gam | Expresses dead Cas9 (dCas9)-CBE4-gam |
124450 | pSB700 HL1gR4 | Expresses gRNA HL1 gR4 in the pSB700 backbone |