Designing a green fluorogenic protease reporter by flipping a beta strand of GFP for imaging apoptosis in animals.

Zhang Q, Schepis A, Huang H, Yang J, Ma W, Torra J, Zhang SQ, Yang L, Wu H, Nonell S, Dong Z, Kornberg TB, Coughlin SR, Shu X
J Am Chem Soc. 2019 Mar 1. doi: 10.1021/jacs.8b13042. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
124494	PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry	Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry
124493	PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry	Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry
124436	pcDNA3-FlipCherry-T2A-GFP	Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells
124434	pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry	Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila
124433	pCS2-GFP1-9	Expresses GFP1-9 in zebrafish
124432	PCS2-FlipGFP(Casp3 cleavage seq)	Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)
124431	PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry	Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish
124430	PCDNA3-GFP1-9 T2A mCherry	Expresses GFP1-9 and T2A mCherry in mammalian cells
124429	PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry	Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells
124428	PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry	Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells