Designing a green fluorogenic protease reporter by flipping a beta strand of GFP for imaging apoptosis in animals.
Zhang Q, Schepis A, Huang H, Yang J, Ma W, Torra J, Zhang SQ, Yang L, Wu H, Nonell S, Dong Z, Kornberg TB, Coughlin SR, Shu X
J Am Chem Soc. 2019 Mar 1. doi: 10.1021/jacs.8b13042.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124428 | PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry | Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells |
| 124429 | PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry | Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells |
| 124430 | PCDNA3-GFP1-9 T2A mCherry | Expresses GFP1-9 and T2A mCherry in mammalian cells |
| 124431 | PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry | Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish |
| 124432 | PCS2-FlipGFP(Casp3 cleavage seq) | Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified) |
| 124433 | pCS2-GFP1-9 | Expresses GFP1-9 in zebrafish |
| 124434 | pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry | Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila |
| 124436 | pcDNA3-FlipCherry-T2A-GFP | Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells |
| 124493 | PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry | Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry |
| 124494 | PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry | Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry |