Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons.
Osaki T, Uzel SGM, Kamm RD
Sci Adv. 2018 Oct 10;4(10):eaat5847. doi: 10.1126/sciadv.aat5847. eCollection 2018 Oct.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 125255 | plenti2-EF1a-ChR2-EYFP-WRPE | 3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activation |
| 125256 | pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE | 3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection |