A Framework for the Modular and Combinatorial Assembly of Synthetic Gene Circuits.
Santos-Moreno J, Schaerli Y
ACS Synth Biol. 2019 Jul 19;8(7):1691-1697. doi: 10.1021/acssynbio.9b00174. Epub 2019 Jun 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124409 | pTU-A-000 | Slot A, empty (step 1) |
| 124410 | pTU-B-000 | Slot B, empty (step 1) |
| 124411 | pTU-C-000 | Slot C, empty (step 1) |
| 124412 | pTU-A-005 | Slot A, contains mKO2-MarA (step 1) |
| 124413 | pTU-B-005 | Slot B, contains sfGFP-MarAn20 (step 1) |
| 124414 | pTU-C-005 | Slot C, contains RepA70-mKate2 (step 1) |
| 124415 | pTU-A-013 | Slot A, empty (step 1) |
| 124416 | pTU-B-013 | Slot B, empty (step 1) |
| 124417 | pTU-C-013 | Slot C, empty (step 1) |
| 124418 | pTU-A-010 | Slot A, contains mKO2-MarA (step 1) |
| 124419 | pTU-B-010 | Slot B, contains sfGFP-MarAn20 (step 1) |
| 124420 | pTU-C-010 | Slot C, contains RepA70-mKate2 (step 1) |
| 124421 | pC-0 | Full MCS (step 2) |
| 124422 | pC-0_v2 | Full MCS (step 2) |
| 124423 | pC-0_v3 | Full MCS (step 2) |
| 124424 | pC-0_v4 | Full MCS, lacks araC & P(BAD) (step 2) |
| 124425 | pC-0_v5 | Full MCS (step 2) |
| 124426 | pC-0_v6 | Full MCS, lacks araC & P(BAD) (step 2) |