Skip to main content
Addgene

Resilient living materials built by printing bacterial spores.

Gonzalez LM, Mukhitov N, Voigt CA
Nat Chem Biol. 2019 Dec 2. pii: 10.1038/s41589-019-0412-5. doi: 10.1038/s41589-019-0412-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
124365pLG163IPTG inducible system (gfpmut2 with Ppsank) for expression in B. subtilis
124366pLG166Gfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilis
124367pLG169Xylose inducible system with the CRE site for expression in B. subtilis
124368pLG173Control plasmid with only spectinomycin
124369pLG231IPTG inducible system (gfpmut2 with Ppsank and riboJ) for expression in B. subtilis
124370pLG320Vanillic acid inducible system for expression in B. subtilis
128409pNM58System with AIP and xylose inducible agrCA sensor
128410pLG371Cuminic acid-inducible system
128411pNM261Vanillic acid inducible production of lysostaphin. LsyT with no signaling peptide for secretion.
128412pNM262Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.
128797pLG327Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

Antibodies from Article