Off-pathway-sensitive protein splicing screening based on a toxin/antitoxin system.

Beyer HM, Iwai H
Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
124352	pBHSTC97	Plasmid expressing NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin
124353	pHBSTC159	Plasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin
124354	pHYSTC299	Plasmid expressing gp41-1 intein inserted into CcdA antitoxin
124355	pHBSTC147	Plasmid expressing inactive gp41-1 intein inserted into CcdA antitoxin
124356	pBHSTC104	Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin
124357	pHBSTC146	Plasmid expressing inactive N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin
124358	pBHDuet40	IPTG-inducible vector encoding the N-terminal gp41-1 split fragment with N-terminally His-tagged GB1
124359	pBHBAD42	Arabinose-inducible vector encoding the C-terminal gp41-1 split fragment fused to GB1