Genome-wide CRISPR screens reveal genetic mediators of cereblon modulator toxicity in primary effusion lymphoma.
Patil A, Manzano M, Gottwein E
Blood Adv. 2019 Jul 23;3(14):2105-2117. doi: 10.1182/bloodadvances.2019031732.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124299 | pLC-Flag-UBE2G1 sg2R-P2A-Hygro | Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site. |
| 124300 | pLC-Flag-SENP8 sg2R-P2A-Hygro | Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site. |
| 124301 | pLC-ZsGreen-P2A-Hygro | Lentiviral vector for expression of ZsGreen-P2A-Hygro casette from a CMV promoter |
| 124302 | pLC-ZsGreen-P2A-Puro | Lentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoter |
| 124303 | pLC-Flag-CRBN-P2A-Hygro | Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter |
| 124304 | pLC-CUL4A WT-P2A-Puro | Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter |
| 124305 | pLC-CUL4A K705R-P2A-Puro | Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter |
| 124306 | pLC-HA-Nedd8-P2A-Hygro | Lentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoter |
| 124307 | pLC-HA active Nedd8-P2A-Hygro | Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter |