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Precise tuning of gene expression levels in mammalian cells.

Michaels YS, Barnkob MB, Barbosa H, Baeumler TA, Thompson MK, Andre V, Colin-York H, Fritzsche M, Gileadi U, Sheppard HM, Knapp DJHF, Milne TA, Cerundolo V, Fulga TA
Nat Commun. 2019 Feb 18;10(1):818. doi: 10.1038/s41467-019-08777-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
124673OVA-miSFIT-2xPerfectTuning expression of cytosolic Ovalbumin coupled to EGFP
124674OVA-miSFIT-1xPerfectTuning expression of cytosolic Ovalbumin coupled to EGFP
124675OVA-miSFIT-8-GTuning expression of cytosolic Ovalbumin coupled to EGFP
124676OVA-miSFIT-19-GTuning expression of cytosolic Ovalbumin coupled to EGFP
124677OVA-miSFIT-17-TTuning expression of cytosolic Ovalbumin coupled to EGFP
124678PD-1-miSFIT-4xTuning expression of PD-1
124679PD-1-miSFIT-1xTuning expression of PD-1
124680PD-1-miSFIT-Ce67Tuning expression of PD-1
124681PD-1-miSFIT-6-C, 16-CTuning expression of PD-1
124682PD-1-miSFIT-1-A, 5-ATuning expression of PD-1
124683PD-1-miSFIT-12-C, 21-GTuning expression of PD-1
124684PD-L1-miSFIT-4xTuning expression of PD-1
124685PD-L1-miSFIT-1xTuning expression of PD-1
124686PD-L1-miSFIT-Ce67Tuning expression of PD-1
124687PD-L1-miSFIT-12-CTuning expression of PD-1
124688PD-L1-miSFIT-9-G, 15-CTuning expression of PD-1
124689PD-L1-miSFIT-6-C, 16-CTuning expression of PD-1
124690EGFP-miSFIT-1xTuning expression of EGFP
124691EGFP-miSFIT-2xTuning expression of EGFP
124692EGFP-miSFIT-9-GTuning expression of EGFP
124693EGFP-miSFIT-12-CTuning expression of EGFP
124694EGFP-miSFIT-6-C,16-CTuning expression of EGFP
124695EGFP-miSFIT-10-G, 15-CTuning expression of EGFP

Antibodies from Article