Sin3A recruits Tet1 to the PAH1 domain via a highly conserved Sin3-Interaction Domain.
Chandru A, Bate N, Vuister GW, Cowley SM
Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.1038/s41598-018-32942-w.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124386 | His-Sin3a-115-212(pL01) | E.Coli produced SIN3A PAH1 purified by FPLC nickel column |
| 124387 | GB1-His-SAP25-126-186(pL91) | E.Coli produced SAP25 SID purified by FPLC nickel column |
| 124388 | GB1-His-TET1-848-952(pL91) | E.Coli produced TET1-848-952 purified by FPLC nickel column |
| 124390 | GB1-His-TET1-878-911(pL91) | E.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel column |
| 124391 | GB1-His-TET1-878-911-I894A(pL91) | E.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel column |
| 124392 | GB1-His-TET1-878-911-L897A(pL91) | E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column |
| 124393 | GB1-His-TET1-878-911-T898A(pL91) | E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column |
| 124394 | GB1-His-TET1-878-911-T898E(pL91) | E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column |
| 124395 | GB1-His-TET1-878-911-L900A(pL91) | E.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel column |
| 124396 | FLAG-FullLength-TET1-WT(21) | Full-length TET1 used to PiggyBAC rescue the function of TET1/2 DKO cells |
| 124397 | FLAG-FullLength-TET1-LLAA(20) | Full-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cells |
| 124398 | FLAG-FullLength-TET1-CXXC(24) | Full-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cells |
| 124399 | FLAG-FullLength-TET1-CXXC/LLAA | Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells |
| 124400 | Gal4-TET1-878-911-WT(pL84) | Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID. |
| 124401 | Gal4-TET1-878-911-L897A(pL84) | Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation. |
| 124402 | Gal4-TET1-878-911-L900A(pL84) | Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation. |
| 124403 | Gal4-TET1-878-911-T898A(pL84) | Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation. |
| 124404 | Gal4-TET1-878-911-I894A(pL84) | Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation. |
| 124406 | FLAG-TET1-746-951(pL72) | TET1-746-951, containing SID, used for Co-IP. |