A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs.

Talas A, Huszar K, Kulcsar PI, Varga JK, Varga E, Toth E, Welker Z, Erdos G, Pach PF, Welker A, Gyorgypal Z, Tusnady GE, Welker E
Nucleic Acids Res. 2021 Jan 15. pii: 6101605. doi: 10.1093/nar/gkaa1220. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
124221	pAT-9208	Cloning plasmid for a self-targeting gRNA library
124222	pAT-9222	Cloning plasmid for EGxFP assay with self-targeting gRNA-s
124225	pAT-9218	Bacterial SpCas9 expression
124226	pAT-9251	Bacterial SpCas9-HF1 expression
124227	pKH-1699	Mammalian SpCas9-HF1 expression