Activity-Related Conformational Changes in d,d-Carboxypeptidases Revealed by In Vivo Periplasmic Forster Resonance Energy Transfer Assay in Escherichia coli.
Meiresonne NY, van der Ploeg R, Hink MA, den Blaauwen T
MBio. 2017 Sep 12;8(5). pii: mBio.01089-17. doi: 10.1128/mBio.01089-17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124215 | mNG-mCh | Cytoplasmic mNeongreen mCherry tandem, ~19 % energy transfer pNM002 - pSAV057-mNG-mCh |
| 124216 | OmpA-mNG-mCh | Periplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mCh |