Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts.
Wang S, Romano FB, Field CM, Mitchison TJ, Rapoport TA
J Cell Biol. 2013 Dec 9;203(5):801-14. doi: 10.1083/jcb.201308001. Epub 2013 Dec 2.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
124063 | pGEX-4T-3-cytXlATL2 | Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail. |
124064 | pGEX-4T-3-cytXlATL2-R232Q | Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL |