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Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.

Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
124166KA0637_pPBCAG-rtTAM2-INpiggyBac-based expression of rtTA-M2
124167KA1775_pPBCAG-rtTAM2-IHpiggyBac-based expression of rtTA-M2
124168KA0717_pPB-hCMV*1-cHA-IRESVenuspiggyBac-based Dox-inducible expression
124169KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2piggyBac-based Dox-inducible expression
124170KA1065_pPB-hCMV*1-cHA-IRESH2BmCherrypiggyBac-based Dox-inducible expression
124171KA0718_pPB-TREtight-cHA-IRESVenuspiggyBac-based Dox-inducible expression
124172KA1123_pPB-TREtight-cHA-IRESH2BmKO2piggyBac-based Dox-inducible expression
124173KA1130_pPB-TREtight-cHA-IRESH2BmCherrypiggyBac-based Dox-inducible expression
124174KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2piggyBac-based Dox-inducible expression (weak)
124175KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2piggyBac-based Dox-inducible expression (very weak)
124176KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2piggyBac-based Dox-inducible expression (moderate)
124177KA1042_pPB-hCMV*1-Esrrb-IVpiggyBac-based Dox-inducible expression of Esrrb
124178KA2880_pPB-TREtight-FLAG-Esrrb-InOpiggyBac-based Dox-inducible expression of FLAG-Esrrb
124179KA2944_pPB-TREtight-Esrrb-FLAG-InOpiggyBac-based Dox-inducible expression of Esrrb-FLAG
124180KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IPpiggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer
124181KA1270_pPB-gPou5f1_3.5kb-UBCmin-IPpiggyBac-based reporter (Puro) driven by the Pou5f1 enhancer
124183KA1153_pPBCAG-MerCreMer-INpiggyBac-based expression of the tamoxifen-inducible Cre
124184KA1158_pPBCAG-MerCreMer-IZpiggyBac-based expression of the tamoxifen-inducible Cre
124185KA1390_pGL4-UBCmin-luc2Luciferase reporter with the UBC minimal promoter
124186KA1395_pGL4-UBCmin-luc2/Klf4-B'Luciferase reporter driven by the Klf4 enhancer
124187KA1396_pGL4-UBCmin-luc2/Klf4-E'Luciferase reporter driven by the Klf4 enhancer
124188KA1401_pGL4-UBCmin-luc2/Klf4-F'Luciferase reporter driven by the Klf4 enhancer
124189KA1402_pGL4-UBCmin-luc2/Nanog-A'Luciferase reporter driven by the Nanog enhancer
124190KA1403_pGL4-UBCmin-luc2/Nanog-B'Luciferase reporter driven by the Nanog enhancer
124191KA1865_pGL4-UBCmin-luc2/Pou5f1_CR4Luciferase reporter driven by the Pou5f1 enhancer
124192KA1866_pGL4-UBCmin-luc2/Pou5f1_CR3Luciferase reporter driven by the Pou5f1 enhancer
124193KA1867_pGL4-UBCmin-luc2/Pou5f1_CR2Luciferase reporter driven by the Pou5f1 enhancer
124194KA1868_pGL4-UBCmin-luc2/Utf1-A'Luciferase reporter driven by the Utf1 enhancer
124195KA1869_pGL4-UBCmin-luc2/Fgf4-A'Luciferase reporter driven by the Fgf4 enhancer
124196KA1870_pGL4-UBCmin-luc2/Esrrb-B'Luciferase reporter driven by the Esrrb enhancer
124197KA1874_pGL4-UBCmin-luc2/Sox2_SRR1Luciferase reporter driven by the Sox2 enhancer
124198KA1875_pGL4-UBCmin-luc2/Sox2_SRR2Luciferase reporter driven by the Sox2 enhancer
124199KA2626_pGL4-UBCmin-luc2/Klf2-D'Luciferase reporter driven by the Klf2 enhancer
124200KA2627_pGL4-UBCmin-luc2/Jam2-A'Luciferase reporter driven by the Jam2 enhancer
124201KA2628_pGL4-UBCmin-luc2/Tbx3-B'Luciferase reporter driven by the Tbx3 enhancer
124202KA2058_pX330A-G2PExpression of SpCas9 and sgRNA
124203KA2601_eSpCas9-G2PExpression of eSpCas9(1.1) and sgRNA
124204KA2706_eSpCas9-PGKPacdtkChExpression of eSpCas9(1.1) and sgRNA
124205KA2963_eSpCas9-PGKHygdtkChExpression of eSpCas9(1.1) and sgRNA

Antibodies from Article