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Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds.

Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou Q, Li W
Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
123632pCAG-ArCas12a-2AeGFPMammalian expression, Genome editing
123633pCAG-BsCas12a-2AeGFPMammalian expression, Genome editing
123634pCAG-HkCas12a-2AeGFPMammalian expression, Genome editing
123635pCAG-LpCas12a-2AeGFPMammalian expression, Genome editing
123636pCAG-PrCas12a-2AeGFPMammalian expression, Genome editing
123637pCAG-PxCas12a-2AeGFPMammalian expression, Genome editing
123638pCAG-AsCas12a-2AeGFPMammalian expression, Genome editing
123639pUC19-U6-crRNA1Mammalian expression, Genome editing, gRNA scaffold
123640pUC19-U6-crRNA4n96Mammalian expression, Genome editing, gRNA scaffold
123641pUC19-U6-tRNAx2Mammalian expression, Genome editing, backbone

Antibodies from Article