Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors.
Grunewald J, Zhou R, Garcia SP, Iyer S, Lareau CA, Aryee MJ, Joung JK
Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123611 | nCas9-UGI-NLS-P2A-EGFP (pJUL1001) | CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1). |
| 123612 | BE3-P2A-EGFP (pJUL977) | CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP. |
| 123613 | BE3(E63Q)-P2A-EGFP (pRZ189) | CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant). |
| 123614 | SECURE BE3(R33A)-P2A-EGFP (pJUL1085) | CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A). |
| 123615 | SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410) | CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A). |
| 123616 | NLS-nCas9-NLS-P2A-EGFP (pRZ70) | CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains). |