Efficient scarless genome editing in human pluripotent stem cells.
Ikeda K, Uchida N, Nishimura T, White J, Martin RM, Nakauchi H, Sebastiano V, Weinberg KI, Porteus MH
Nat Methods. 2018 Dec;15(12):1045-1047. doi: 10.1038/s41592-018-0212-y. Epub 2018 Nov 30.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123661 | pSLHDR01_mCherry_tCD19 | Construction of 1st donor for scarless genome editing |
| 124061 | pSLHDR02_EGFP_tCD8 | Construction of 1st donor for scarless genome editing |
| 124062 | pSLHDR03_mCherry_tCD19_unique guide | Construction of 1st donor for scarless genome editing with unique guide |
| 124209 | pSLHDR_R1 | gRNA/Cas9 expression for 2nd editing using pSLHDR01, 02 |
| 124211 | pSLHDR_R4 | gRNA/Cas9 expression for 2nd editing using pSLHDR04 |