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Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania.

Zhang WW, Matlashewski G
mSphere. 2019 Aug 21;4(4). pii: 4/4/e00408-19. doi: 10.1128/mSphere.00408-19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
123261pLdSaCNExpresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania
123262pLPhygSaCas9Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania
123265pSPneoSagRNAHExpresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania
123266pNeoSagRNAHExpresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Antibodies from Article