EASI Transformation: An Efficient Transient Expression Method for Analyzing Gene Function in Catharanthus roseus Seedlings.
Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, Lee-Parsons CWT
Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. eCollection 2019.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123180 | pSB63 - pL0_fLUC-I (CDS1) | Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants |
| 123181 | pSB123 - pL0_rLUC-I (CDS1) | Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants |
| 123182 | pSB65 - pL0_tGFP-I (CDS1) | Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014) |
| 123183 | pSB61 - pL0_V2 (CDS1) | Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005 |
| 123184 | pSB139 - pL0_ZCT1 (CDS1) | Golden Gate (MoClo; CDS1) compatible ZCT1 gene from Catharanthus roseus; a repressor of the STR1 promoter from C. roseus |
| 123185 | pSB142 - pL0_ORCA3 (CDS1) | Golden Gate (MoClo; CDS1) compatible ORCA3 gene from Catharanthus roseus; an activator of the STR1 promoter from C. roseus |
| 123186 | pSB62 - pL0_STR (pro + 5U) | Golden Gate (MoClo; PRO + 5U) compatible STR1 promoter from Catharanthus roseus |
| 123187 | pSB90 - pAGM4723_VirGN54D | Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria |
| 123188 | pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS | binary plant vector for transient fLUC (with intron) expression |
| 123189 | pSB97 - pL2_pVS1_2x35S::fLUC-I::tNOS | binary plant vector for transient fLUC (with intron) expression |
| 123190 | pSB115 - pL2_pSB90_fLUC-I_tGFP-I | binary plant vector for transient tGFP (with intron) and fLUC (with intron) expression |
| 123191 | pSB116 - pL2_pSB90_fLUC-I_P19 | binary plant vector for transient TBSV P19 and fLUC (with intron) expression |
| 123192 | pSB117 - pL2_pSB90_fLUC-I_V2 | binary plant vector for transient TYLCV V2 and fLUC (with intron) expression |
| 123193 | pSB118 - pL2_pSB90_fLUC-I_HcPro | binary plant vector for transient TEV HcPro and fLUC (with intron) expression |
| 123194 | pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1 | binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression |
| 123195 | pSB153 - pL2_pSB90_2x35S::ZCT1::tMAS | binary plant vector for transient expression of ZCT1 from Catharanthus roseus |
| 123196 | pSB160 - pL2_pSB90_2x35S::ORCA3::tMAS | binary plant vector for transient expression of ORCA3 from Catharanthus roseus |
| 123197 | pSB161 - pL2_pSB90_2x35S::GUS::tMAS | binary plant vector for transient expression of GUS (with introns) |
| 123198 | pSB165 - pL1R3_pMAS::rLUC-I::tMAS | binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) |
| 123199 | pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS | binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) |
| 123200 | pSB167 - pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMAS | binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in plants |