Can asymmetric post-translational modifications regulate the behavior of STAT3 homodimers?
Letra-Vilela R, Cardoso B, Silva-Almeida C, Maia Rocha A, Murtinheira F, Branco-Santos J, Rodriguez C, Martin V, Santa-Marta M, Herrera F
FASEB Bioadv. 2020 Jan 27;2(2):116-125. doi: 10.1096/fba.2019-00049. eCollection 2020 Feb.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123164 | Venus1-WTSTAT3 | Expresses wild type STAT3 fused to the 1-157 fragment of Venus in mammalian cells |
| 123165 | Venus2-WTSTAT3 | Expresses wild type STAT3 fused to the 158-238 fragment of Venus in mammalian cells |
| 123166 | Venus1-K49R-STAT3 | Expresses K49R STAT3 fused to the 1-157 fragment of Venus in mammalian cells |
| 123167 | Venus2-K49R-STAT3 | Expresses K49R STAT3 fused to the 158-238 fragment of Venus in mammalian cells |
| 123168 | Venus1-K140R-STAT3 | Expresses K140R STAT3 fused to the 1-157 fragment of Venus in mammalian cells |
| 123169 | Venus2-K140R-STAT3 | Expresses K140R STAT3 fused to the 158-238 fragment of Venus in mammalian cells |
| 123170 | Venus1-K685R-STAT3 | Expresses K658R STAT3 fused to the 1-157 fragment of Venus in mammalian cells |
| 123171 | Venus2-K685R-STAT3 | Expresses K695R STAT3 fused to the 158-238 fragment of Venus in mammalian cells |
| 123172 | Venus1-Y705F-STAT3 | Expresses Y705F STAT3 fused to the 1-157 fragment of Venus in mammalian cells |
| 123173 | Venus2-Y705F-STAT3 | Expresses Y705F STAT3 fused to the 158-238 fragment of Venus in mammalian cells |
| 123174 | Venus1-S727A-STAT3 | Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells |
| 123175 | Venus2-S727A-STAT3 | Expresses S727A STAT3 fused to the 158-238 fragment of Venus in mammalian cells |
| 123176 | Venus1-Y705F/S727A-STAT3 | Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells |
| 123177 | Venus2-Y705F/S727A-STAT3 | Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells |
| 123178 | Venus1-DeltaCT-STAT3 | Expresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 1-157 fragment of Venus in mammalian cells |
| 123179 | Venus2-DeltaCT-STAT3 | Expresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 158-238 fragment of Venus in mammalian cells |