Deciphering and engineering chromodomain-methyllysine peptide recognition.
Hard R, Li N, He W, Ross B, Mo GCH, Peng Q, Stein RSL, Komives E, Wang Y, Zhang J, Wang W
Sci Adv. 2018 Nov 7;4(11):eaau1447. doi: 10.1126/sciadv.aau1447. eCollection 2018 Nov.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138250 | pcDNA3-CBX1mut-Dronpa | Triple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1. |
| 138251 | pcDNA3-CBX1mut-PA-mCherry | Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1. |
| 138252 | pcDNA3-CBX1wt-PA-mCherry | Wild-type CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). |