Luminescent kinase activity biosensors based on a versatile bimolecular switch.
Herbst KJ, Allen MD, Zhang J
J Am Chem Soc. 2011 Apr 20;133(15):5676-9. doi: 10.1021/ja1117396. Epub 2011 Mar 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138202 | pDNA3-Cerulean-FHA1-NES | Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted. |
| 138203 | pcDNA3-PKAsub-YPet-NES | Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES. |
| 138205 | pcDNA3-PKAsub(T/A)-YPet-NES | Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES. |
| 138206 | pcDNA3-PKAsub-YPet-Kras | Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES. |
| 138207 | pcDNA3-PKCsub-YPet-NES | Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES |
| 138208 | pcDNA3-PKCsub(T/A)-YPet-NES | Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES |
| 138210 | pcDNA3-RLuc8C-FHA1-NES | Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted. |
| 138211 | pcDNA3-mCherry-PKAsub-RLuc8N-NES | Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES. |
| 138212 | pcDNA3-PKAsub(T/A)-RLuc8N-NES | Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES. |
| 138213 | pcDNA3-mCherry-PKCsub-RLuc8N-NES | Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES. |
| 138299 | pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES | Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES. |