High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases.

Bessen JL, Afeyan LK, Dancik V, Koblan LW, Thompson DB, Leichner C, Clemons PA, Liu DR
Nat Commun. 2019 Apr 26;10(1):1937. doi: 10.1038/s41467-019-09987-0. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
123137	pBT100-neo	Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.
123136	pCALNL-BsaI-EGFP	Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.
123135	pCMV-Brec1	Mammalian expression vector for Brec1.
123134	pCMV-Tre	Mammalian expression vector for Tre.
123133	pCMV-Cre	Mammalian expression vector for wild-type Cre.
123132	pET-Bxb1-His	Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.
123131	pET-His-VCre	Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag.
123130	pET-His-Dre	Bacterial expression plasmid for wild-type Dre with an N-terminal His-tag.
123129	pET-His-Tre	Bacterial expression plasmid for Tre with an N-terminal His-tag.
123128	pET-His-Cre	Bacterial expression plasmid for wild-type Cre with an N-terminal His-tag.