High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases.
Bessen JL, Afeyan LK, Dancik V, Koblan LW, Thompson DB, Leichner C, Clemons PA, Liu DR
Nat Commun. 2019 Apr 26;10(1):1937. doi: 10.1038/s41467-019-09987-0.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123128 | pET-His-Cre | Bacterial expression plasmid for wild-type Cre with an N-terminal His-tag. |
| 123129 | pET-His-Tre | Bacterial expression plasmid for Tre with an N-terminal His-tag. |
| 123130 | pET-His-Dre | Bacterial expression plasmid for wild-type Dre with an N-terminal His-tag. |
| 123131 | pET-His-VCre | Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag. |
| 123132 | pET-Bxb1-His | Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag. |
| 123133 | pCMV-Cre | Mammalian expression vector for wild-type Cre. |
| 123134 | pCMV-Tre | Mammalian expression vector for Tre. |
| 123135 | pCMV-Brec1 | Mammalian expression vector for Brec1. |
| 123136 | pCALNL-BsaI-EGFP | Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters. |
| 123137 | pBT100-neo | Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters. |