CasX enzymes comprise a distinct family of RNA-guided genome editors.
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA
Nature. 2019 Feb 4. pii: 10.1038/s41586-019-0908-x. doi: 10.1038/s41586-019-0908-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123120 | pBLO 64.1 | E. coli expression vector for active deltaproteobacteria CasX |
| 123121 | pBLO ddd64.1 | E. coli expression vector for catalytically inactivated deltaproteobacteria CasX |
| 123122 | pBLO2 casX | E. coli expression vector for CasX sgRNA |
| 123123 | pBLO 62.4 | Human expression vector for deltaproteobacteria CasX and sgRNA |
| 123124 | pBLO 62.5 | Human expression vector for planctomyces CasX and sgRNA |
| 126418 | pET 2C-T10-dCasX | E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification |
| 126419 | PBLO CasX-gRNA | E. coli vector for CasX gRNA --- Used for In Vitro Transcription |