A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission.
Liu Q, Sinnen BL, Boxer EE, Schneider MW, Grybko MJ, Buchta WC, Gibson ES, Wysoczynski CL, Ford CP, Gottschalk A, Aoto J, Tucker CL, Kennedy MJ
Neuron. 2019 Jan 15. pii: S0896-6273(19)30003-0. doi: 10.1016/j.neuron.2019.01.002.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122971 | pQL47 EGFP-VAMP-GST | Reporter of botulinum toxin B cleavage |
| 122981 | pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLID | AAV plasmid with human synapsin promoter driving synaptophysin fused to GFP, iLID(V416I) and BoNT/B amino acids 1-146. Co-express with SSPB-BoNT(147-441, Y365A) for vPA-BoNT |
| 122982 | pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID | AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT |
| 122985 | pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A) | AAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT |
| 122986 | pAAV hSyn mCh-IRES-SspB(micro)-BoNT/B(147-441, Y365A) | AAV plasmid with human synapsin promoter driving mCh and SspB(micro)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT |