Design of a Split Intein with Exceptional Protein Splicing Activity.

Stevens AJ, Brown ZZ, Shah NH, Sekar G, Cowburn D, Muir TW
J Am Chem Soc. 2016 Feb 24;138(7):2162-5. doi: 10.1021/jacs.5b13528. Epub 2016 Feb 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
122949	pET-30-His6-SUMO-AEY-CfaN	Bacterial expression of N-intein
122950	pTXB1-CfaC-Gyra	Bacterial expression of C-intein
122951	HC-CfaN	Mammalian codon optimized N-intein