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CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification.

Oakes BL, Fellmann C, Rishi H, Taylor KL, Ren SM, Nadler DC, Yokoo R, Arkin AP, Doudna JA, Savage DF
Cell. 2019 Jan 10;176(1-2):254-267.e16. doi: 10.1016/j.cell.2018.11.052. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
121656pCF204U6-sgRNA EFS-Cas9-wt-P2A-Puro
121658pCF704U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro
121662pCF711U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro
121663pCF712U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro
121665pCF713U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro
121667pCF226EFS-Cas9-wt-P2A-Puro
121668pCF730EF1a-ProCas9-Flavi-P2A-Puro
121669pCF221U6-sgRNA EF1a-mCherry
121685pCF708EF1a-dTEV-protease-mTagBFP2
121891pCF709EF1a-ZIKV-protease-mTagBFP2
121892pCF710EF1a-WNV-protease-mTagBFP2
121893pCF736EF1a-dTEV-protease-GFP
122032pCF738EF1a-WNV-protease-GFP

Antibodies from Article