CRISPR-based tools for targeted transcriptional and epigenetic regulation in plants.
Lee JE, Neumann M, Duro DI, Schmid M
PLoS One. 2019 Sep 26;14(9):e0222778. doi: 10.1371/journal.pone.0222778. eCollection 2019.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122835 | pSC142 | SUC2 promoter with GreenGate A-B flanking sequences |
| 122836 | pMH220 | dCas9 with GreenGate B-C flanking sequences |
| 122837 | pMH186 | MS2-NLS (no stop codon) with GreenGate C-D flanking sequences |
| 122838 | pEF003 | RBCS terminator with GreenGate C-D flanking sequences |
| 122839 | pMH185 | VP64 activator domain with GreenGate D-E flanking sequences |
| 122840 | pDG058 | SRDX repressor domain with GreenGate D-E flanking sequences |
| 122841 | pJL023 | p300 core domain with GreenGate D-E flanking sequences |
| 122854 | pJL035 | G9a SET domain with GreenGate D-E flanking sequences |
| 122855 | pJL032 | KYP SET domain with GreenGate D-E flanking sequences |
| 122856 | pMH183 | MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences |
| 122857 | pMH184 | MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences |
| 122859 | pJL003 | Binary expression vector containing 35S:MS2-GFP:tRBCS followed by GreenGate A-G sequences for insertion of additional modules |
| 122860 | pMH204 | Binary expression vector containing 35S:MS2-VP64:tRBCS followed by GreenGate A-G sequences for insertion of additional modules |
| 122861 | pMH205 | Binary expression vector containing pSUC2:MS2-VP64:tRBCS followed by GreenGate A-G sequences for insertion of additional modules |
| 122862 | pJL009 | Binary expression vector containing 35S:MS2-SRDX:tRBCS followed by GreenGate A-G sequences for insertion of additional modules |
| 122863 | pJL024 | Binary expression vector containing 35S:MS2-p300:tRBCS followed by GreenGate A-G sequences for insertion of additional modules |
| 122864 | pJL036 | Binary expression vector containing 35S:MS2-G9a:tRBCS followed by GreenGate A-G sequences for insertion of additional modules |
| 122865 | pJL042 | Binary expression vector containing 35S:MS2-KYP:tRBCS followed by GreenGate A-G sequences for insertion of additional modules |