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Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella.

Shaw JA, Bourret TJ
J Vis Exp. 2019 May 13;(147). doi: 10.3791/59630. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
122702pSKAPDesigned for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.
122703pSKAP_AApSKAP plasmid containing the unique DNA barcode "AA" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122704pSKAP_ABpSKAP plasmid containing the unique DNA barcode "AB" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122705pSKAP_ACpSKAP plasmid containing the unique DNA barcode "AC" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122706pSKAP_ADpSKAP plasmid containing the unique DNA barcode "AD" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification
122707pSKAP_AEpSKAP plasmid containing the unique DNA barcode "AE" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification
122708pSKAP_AFpSKAP plasmid containing the unique DNA barcode "AF" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122709pSKAP_AGpSKAP plasmid containing the unique DNA barcode "AG" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122710pSKAP_AHpSKAP plasmid containing the unique DNA barcode "AH" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122711pSKAP_AIpSKAP plasmid containing the unique DNA barcode "AI" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122712pSKAP_AJpSKAP plasmid containing the unique DNA barcode "AJ" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122713pSKAP_AKpSKAP plasmid containing the unique DNA barcode "AK" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122714pSKAP_ALpSKAP plasmid containing the unique DNA barcode "AL" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122715pSKAP_AMpSKAP plasmid containing the unique DNA barcode "AM" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification
122716pSKAP_ANpSKAP plasmid containing the unique DNA barcode "AN" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122717pSKAP_AOpSKAP plasmid containing the unique DNA barcode "AO" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122718pSKAP_APpSKAP plasmid containing the unique DNA barcode "AP" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122719pSKAP_AQpSKAP plasmid containing the unique DNA barcode "AQ" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification
122720pSKAP_ARpSKAP plasmid containing the unique DNA barcode "AR" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122721pSKAP_ASpSKAP plasmid containing the unique DNA barcode "AS" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122722pSKAP_ATpSKAP plasmid containing the unique DNA barcode "AT" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122723pSKAP_AUpSKAP plasmid containing the unique DNA barcode "AU" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122724pSKAP_AVpSKAP plasmid containing the unique DNA barcode "AV" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122725pSKAP_AWpSKAP plasmid containing the unique DNA barcode "AW" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
122726pSKAP_AXpSKAP plasmid containing the unique DNA barcode "AX" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Antibodies from Article