Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells.
Nakamura M, Srinivasan P, Chavez M, Carter MA, Dominguez AA, La Russa M, Lau MB, Abbott TR, Xu X, Zhao D, Gao Y, Kipniss NH, Smolke CD, Bondy-Denomy J, Qi LS
Nat Commun. 2019 Jan 14;10(1):194. doi: 10.1038/s41467-018-08158-x.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123657 | pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4 | DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation. |
| 123658 | pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4 | DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter. |
| 125148 | pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4 | Lentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter. |