Skip to main content
Addgene

Continuous evolution of base editors with expanded target compatibility and improved activity.

Thuronyi BW, Koblan LW, Levy JM, Yeh WH, Zheng C, Newby GA, Wilson C, Bhaumik M, Shubina-Oleinik O, Holt JR, Liu DR
Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
122595pBT2dR3-proB BE PACE selection, express T7 RNA polymerase with degron-GTCCA target
122596pBT13-splitC clone M13 phage by SapI Golden Gate
122597pBT13-splitD clone M13 phage by SapI Golden Gate
122598pBT29-splitC clone M13 phage by SapI Golden Gate
122599pBT29-splitD clone M13 phage by SapI Golden Gate
122600pBT44c BE PACE selection, express C-intein-Cas9-ugi fusion
122601pBT100.106b clone APOBEC1 phage by SapI Golden Gate
122602pBT100.110 clone CDA1 phage by SapI Golden Gate
122603pBT100.136c clone FERNY phage by SapI Golden Gate
122604pBT120b BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA
122605pBT137-splitC clone M13 phage by SapI Golden Gate
122606pBT137-splitD clone M13 phage by SapI Golden Gate
122607pBT276 express CDA1-BE4max in mammalian cells
122608pBT277 express evoCDA1-BE4max in mammalian cells
122609pBT279 express FERNY-BE4max in mammalian cells
122610pBT280 express evoFERNY-BE4max in mammalian cells
122611pBT281 express evoAPOBEC1-BE4max in mammalian cells
125612pBT371Expresses CDA1-BE4max-NG in mammalian cells
125613pBT372Expresses evoCDA1-BE4max-NG in mammalian cells
125614pBT373Expresses FERNY-BE4max-NG in mammalian cells
125615pBT374Expresses evoFERNY-BE4max-NG in mammalian cells
125616pBT375Expresses evoAPOBEC1-BE4max-NG in mammalian cells
125617pBT376Expresses BE4max-NG in mammalian cells
127843pBT195clone BE4max CBEs by SapI Golden Gate
127844pBT362clone BE4max-NG CBEs by SapI Golden Gate
127845pBT271.359clone A3A CBEs by SapI Golden Gate
127846pBT271.361clone A3A-W98Y CBEs by SapI Golden Gate

Antibodies from Article