Single-color, ratiometric biosensors for detecting signaling activities in live cells.

Ross BL, Tenner B, Markwardt ML, Zviman A, Shi G, Kerr JP, Snell NE, McFarland JJ, Mauban JR, Ward CW, Rizzo MA, Zhang J
eLife. 2018 Jul 3;7. pii: 35458. doi: 10.7554/eLife.35458 (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
123355	pcDNA3-Venus-cpVenus-FLARE-ICUE	Yellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
123354	pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER	Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.
123353	pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon	Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.
123352	pcDNA3-mCherry-mCherry-FLARE-Cameleon	Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.
123351	pcDNA3-Venus-Venus-FLARE-Cameleon	Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.
123350	pcDNA3-Venus-cpVenus-FLARE-Cameleon	Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
123348	pcDNA3-CKAR2	Improved FRET-based biosensor for monitoring Protein Kinase C activity.
123346	pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR	Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.
123345	pcDNA3-mCherry-mCherry-FLARE-CKAR	Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.
123344	pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)	Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.